Determine the isoelectric point of the following peptide. show your work! hints. you will need to determine the two pk groups that will be used to calculate the pi. start by drawing peptide in its most acidic form. begin removing protons from the one with the lowest pk values until you receive form where net charge is zero. use pk values of the last group the proton was removed from and the pk of the next group in line to be removed to calculate the pl h; n-glu-lys-leu-asp-glu-his-cooh