Amixture containing four proteins with native molecular weights: 120 kda, 100 kda, 40 kda, and 10 kda were applied on a size-exclusion chromatography column (1 cm (diameter) x 40 cm) with a fractionation range of 10-60 kda. the bed volume is 31.42 ml, and the void volume is 10.47 ml.
(a) sketch a chromatogram representing the separation of this mixture of proteins with the above mentioned size exclusion column. label the y-axis "arbitrary absorbance at 280nm" and the x-axis "volume of elution". have the x-axis with tick marks at 10, 20, 30 and 40 ml of elution. label the contents of each peak.