Biology, 26.07.2019 06:00 blacee01

Is defined or complete media used to grow the colonies to be stamped on the velvet?

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A culture of bacteiral cells are spread on a petri plate of complete medium. Both prototrophs and auxotrophs grow on the medium and their colonies appear in a random arrangement. Piece of sterilized velvet is pressed on this plate of colonies, and the velvet pickus up some cells form each colony. The velvet can then be pressed on a number of petri plates, whcih reproduces the same pattern of bacterial colonies. One of these replica plates contains minimal media, and each of the remaining replica plates contain minimal medium supplemented with a different compound. A cplony that grows on minimal media represents a protoroph, whereas a colony tha fails to grow on minimal medium represents an auxotroph.REPLICA PLATING CONTINUEDAn autotroph that grows on a supplemented minimal medium plate is unable to synthesize either the supplemented compound or a precursor to that compound. the indiviulal clonies of interest can then be isolated and grown out.

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fertilization, pictured in figure 24.23a is the process in which gametes (an egg and sperm) fuse to form a zygote. the egg and sperm each contain one set of chromosomes. to ensure that the offspring has only one complete diploid set of chromosomes, only one sperm must fuse with one egg. in mammals, the egg is protected by a layer of extracellular matrix consisting mainly of glycoproteins called the zona pellucida. when a sperm binds to the zona pellucida, a series of biochemical events, called the acrosomal reactions, take place. in placental mammals, the acrosome contains digestive enzymes that initiate the degradation of the glycoprotein matrix protecting the egg and allowing the sperm plasma membrane to fuse with the egg plasma membrane, as illustrated in figure 24.23b. the fusion of these two membranes creates an opening through which the sperm nucleus is transferred into the ovum. the nuclear membranes of the egg and sperm break down and the two haploid genomes condense to form a diploid genome.

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Answer from: Quest

A. radioactive dating

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