You are trying to purified two versions of protein a.1- the wild-type (nonmutated) protein and 2- a mutant version with a 3 amino acids substitution. you treated both samples the exact way and passed them through ion-exchange column (with positive matrix). you noticed that the mutant protein of a runs through the column more slowly than the wild type. what changes in the mutant protein is most likely to explain this result?

Salmonella typhimurium is a food-borne bacterial pathogen that can cause severe gastrointestinal disease. salmonella is often phagocytosed by macrophages that normally destroy bacterial pathogens, but this bacterium is somehow able to prevent the complete maturation of phagolysosomes. salmonella is able to tolerate the low ph of phagolysosomes, and in fact responds to the drop in ph by secreting toxins that interfere with other cellular responses. which of the following differences would you expect to contribute to the survival of a bacterium within an salmonella-containing phagosome, compared to a healthy phagolysosome? decreased activity of acid hydrolases increased activity of acid hydrolases decreased import of h+ ions increased import of h+ ions my guess is decreased activity of acid hydrolases but i'm not sure.

Which of the following is an example of competition that could be found in a forest? question 14 options: deer eat berries and their droppings seed new areas creating more berry bushes two fox populations utilize the same rabbit population as their main food source two hawk populations utilize the same scorpion population as their main food source lack of sunlight due to changes in the seasons restricts the growth of certain plants

Why does sexual reproduction result in more genetic variation in a species