Nitrogen fixation is carried out primarily by

1. Bacteria

Nitrogen fixation is carried out primarily by organism –bacteria. Further, some of these bacteria can synthesis and change N2 and form ammonia in the said procedure, now called as nitrogen fixation. These organisms –bacteria- are associated with a symbiotic relationship and free-living with either other organisms such as protozoa, termites or plants. This conversion happens in accord with the mutualistic relationship the bacteria has with its host and undergoes during this process nitrogenase is present.    

When scientist examine data such as historical documents, they are using the "scientific methods"