3. (3 pts) The H295R cells for the final experiment were treated with Atrazine using the following protocol:
Step 1: Combine
1 ul of (3 X 10 ppm Atrazine, DMSO)
0.999 ml of cell culture media
Step 2: Combine the following 3 liquids (consider the final volume of this mixture
= 60 ml for your calculations).
60 ul of the solution prepared in step 1
3 ml of cells suspended in cell culture media.
57 ml of cell culture media
a. What is the concentration of Atrazine in ppb in the treated cells?
b. How much more concentrated is the atrazine in the highlighted solution than in the
treated cells? Describe the highlighted solution (relative to the experiment) in X terms.
C. What is the concentration of DMSO in the highlighted solution in %v/v?
d. What is the concentration of DMSO in the treated cells in %v/v units?