Why it is difficult to detect Legionella pneumophila using gram stain microscopy?

Explanation:

While L. pneumophila is categorized as a Gram-negative organism, it stains poorly due to its unique lipopolysaccharide content in the outer leaflet of the outer cell membrane.

Failure to diagnose Legionnaires' disease is largely due to a lack of clinical awareness. In addition, legionellae, the bacteria that cause this disease, are fastidious and not easily detected. Legionellae are gram-negative bacteria found in freshwater environments.

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