Sanger’s chain termination sequencing is the method that has been used most often in the last 40 years. Which statement about it is true? The reactions incorporate dideoxy nucleotide triphosphates (ddNTPs) throughout the growing nucleotide chain during extension by the polymerase.
The reactions terminate extension with a radioactively or fluorescently labeled dNTP.
All 4 G, A, T, and C reactions can be run in the same tube as long as each ddNTP has a different “colored” tag (a tag that can be read at a different wavelength)
The reactions are run in miniaturized, massively parallel flow cells to get maximum read lengths.