Prior to the discovery of Nanog, it was already known that Oct4 is not required to make a blastocyst, but is required for correct differentiation of the blastocyst. Design an experiment that would test whether Nanog expression requires Oct4 function in an intact embryo. Required:
Clearly state your experimental procedures, and your expectations if in fact Nanog expression depends on Oct4 function.

the level of progesterone is low at the pre-ovulatory phase of the cycle but it gradually increases and the levels are high at the luteal phase. if pregnancy occurs, the level of progesterone will be high throughout the pregnancy.

if egg is fertilized and the mother gets pregnant, progesterone starts preparing the uterus for the implantation. it starts building up the lining of uterus and make it hostile of the period of pregnancy.

when you breathe in, or inhale, your diaphragm contracts (tightens) and moves downward. this increases the space in your chest cavity, into which your lungs expand. the intercostal muscles between your ribs also enlarge the chest cavity. they contract to pull your rib cage both upward and outward when you inhale.