You are attempting to carry out a transposon mutagenesis experiment using the pRL27 system. The Donor strain you use was giving to you by a lab mate who has been culturing it continuously for several months. When you set up your control plates, you find that the Donor strain grows poorly on Luria DAP Agar. Your transformation efficiency is very poor for this attempt at mutageneis. What likely happened to the Donor strain

They don’t store and transmit heredity

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Here is your answer:                                                                                                                                                                                                                                                   formula for volume: base*width*height=                                                                                                 5*2*10=100                                                                                   your answer is 100cm.