4. Nebulin is an actin-binding protein that can be found in skeletal muscles. Nebulin is large in size and functions to stabilize actin and regulate muscle contractions. The gene that codes for nebulin, NEB, is on chromosome 2 in
humans. Mutations to the NEB gene can result in mutations in the amino acid sequence of the resulting nebulin
protein. If the mutation in the gene causes an amino acid deletion in the sequence of amino acids that make up
nebulin, how will this affect the structure of nebulin?
a. Only the primary structure of Nebulin will be affected; the secondary and tertiary structures will not be
affected.
b. Both the primary structure and secondary structure of nebulin will be affected, while the tertiary form will
not be affected.
c. The primary, secondary, and tertiary structures of nebulin will be affected.
d. An amino acid deletion would not affect the structure of nebulin.

americans began to notice growing differences between the northern and southern sections of the country. there were more people living in cities in the north, which made it more urban. more people lived on farms in the south, which made it more rural. while many people were farmers in the northern states, there were now other ways to make a living, such as working in mechanical cotton gins factories manufacturing cloth, tools and other goods. people in the north supported high tariffs on goods imported from europe because the extra cost encouraged people to “buy american” instead. in the southern states, planters did not like tariffs because they sold most of their production to europe and bought their tools from there as well. southerners believed the protective tariff was something that only northern factory owners keep their profits high. so the southern politicians came up with an idea. they would opt out. they would simply ignore the protective tariff. it was ‘null and void’ in south carolina. the state would not collect the taxes on goods imported into south carolina, no matter what the president said they had to do. this decision led to the nullification crisis.