A student carried out an investigation using amylase from a saprophytic fungus. Six wells (holes) of the same size were cut in a starch agar plate. Each well was filled with the same
concentration and volume of amylase solution. An equal volume of buffer solution was added to
produce a different pH in each well. The plates were incubated at 25°C for 4 hours and then
covered with iodine solution. It was observed that there were clear rings around each well. The
width of these clear rings is shown in the table.
pH
Width of clear ring
/ mm
4
1
5
2
6
6
7
11
8
9
9
3
i)
What conclusion can be drawn from the results?
1) Use an appropriate method to estimate the maximum rate of reaction that was observed in
this investigation. Show your working
Maximum rate of reaction