The enzyme machinery responsible for mismatch repair must be able to distinguish between the template strand and a newly synthesized strand. How is that achieved in a bacterial cell, such as E. coli

For a short period of time after replication, the sequence GATC is methylated at the A only on the template strand.

thymine. in rna, thymine is replace by uracil

well we can't tell you because we cant see the time give a screen shot or something.