1) Why does the addition of dideoxynucleotides to the DNA sequencing reaction cause DNA synthesis to stop after the modified nucleotide is added?
A)Because once a modified nucleotide is incorporated into the DNA, there is no free 5'OH group to attach the next nucleotide being added.
B)Because once a modified nucleotide is incorporated into the DNA, there is no free 3'PO4-group to attach the next nucleotide being added.
C)Because once a modified nucleotide is incorporated into the DNA, there is no free 3'OH group to attach the next nucleotide being added.
D)Because once a modified nucleotide is incorporated into the DNA, there is no free 5'PO4-group to attach the next nucleotide being added.
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