In a leaf disc assay disc float in solution because

step 1 ammonium can be absorbed by plants, but some is converted into nitrates which are better for plants to absorb. this is called nitrification. 2. step 2 the deer eats the plants and uses the nitrogen-containing proteins for cell growth. 3. step 3 bacteria in the soil conduct nitrogen fixation to convert nitrogen gas from the atmosphere into ammonium. 4. step 4 some of the nitrates are absorbed back into plants, but denitrification breaks down the nitrates into nitrogen gas released back into the atmosphere. 5. step 5 nitrogen is found in the urine, which gets broken down into ammonium through the process of ammonification. 6. step 6 plants absorb the ammonium and begin assimilation, the process to add the nitrogen to protein.

the answer to your question is slate

the principles and empirical processes of discovery and demonstration considered characteristic of or necessary for scientific investigation, generally involving the observation of phenomena, the formulation of a hypothesis concerning the phenomena, experimentation to test the hypothesis, and development of a conclusion that confirms, rejects, or modifies the hypothesis.