he purpose of this discussion to teach in practice, how you calculate the number of bacteria in a broth culture of a bacterium. Also, the knowing how a specific bacteria species grows in the laboratory conditions and determination of its growth curve allows studying effects of various chemical and physical factors on their growth. So, it is customary to find some basic growth characteristics of a bacterium for research. Q1. In your opinion, how can you use information gathered from growth curve measurements? Give some examples. Q2. Give an equation for measuring bacteria doubling time and show how you use it with an example. Q3. List various steps in making a bacterial growth curve. Q4. Do you think culturable bacteria isolated from an environment and in laboratory condition will have the same doubling time. Why/Why not? Explain.