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tsd is temerature dependent sex determination.

tsd: it is a type of environmental sex determination in which the temperature experienced during embryonic/ larval development (ambient temperature for development) determine the sex of the offspring.

crocodile displays the tsd where the ambient temperature of the developing egg determines the individual's sex. for example in the american alligator's eggs, incubation at 33 ºc produces mostly males, while incubation at 30 ºc produces mostly females. so if the american alligator's egg will get the ambient temperature (33 degree celcius)during embryonic development from egg, the offspring will be male and same for the female sex determination.

during the process of dna replication, brdu can replace thymidine and incorporate into the newly synthesized dna, cell proliferation thus can be reflected in the extent of brdu incorporation.