Below is the sequence of the HEXA gene with the underlined portion the 1278TATC allele that recessively caused Tay-Sachs disease. Answer the following questions about this sequence: 5’ 3’

3’ 5’

a. Identify all the PAM sites by clearly marking them in the sequence above.
b. Pick one that is appropriate for the goal of removing the 4 extra TATC nucleotides. There are multiple good answers and you should only choose one.
c. Design a 20bp sgRNA spacer sequence that will make an appropriate cut. There are multiple good answers, and you should only choose one. Be sure to label 5’ and 3’ and the side that would attach to the scaffold sequence.
d. Would you use an HDR template or NHEJ to consistently make the correct edit? Explain

Can someone pretty me with this ? it’s urgent.compare the three theories of emotion. here are the three theories of emotion. cannon-bard theory of emotion: the belief that both psychological arousal and emotional experience are produced stimultaneously ( at the same time) by the same nerve stimulus. james-lange theory of emotion: the belief that emotional experience is a reaction to bodily events occurring as a result of an external situation. ( “ i feel sad because i am crying.) schachter-singer theory of emotion: the belief that emotions are jointly determined by a nonspecific kind of psychological (bodily function) arousal and its interpretation, based on environmental ( natural surroundings) cues. which of these theories makes most sense to you? why. your response should be written using proper english spelling and grammar. and it needs to be 10 complete sentences.

The process of meiosis produces only gametes, eggs or sperm. according to the diagram, how many sperm are formed from each cell that undergoes meiosis?

Abird flies by moving its wings up and down. which two systems are most directly responsible for the movement of a bird's wings? a. skeletal and muscular systems b. respiratory and muscular systems c. skeletal and digestive systems d. respiratory and digestive systems