You want to use RNA interference to make a fruit fly that lacks the function of gene X only in its antenna - the rest of the fly's body has a functional gene X. Which of the following things would you need to construct an RNAi transgene for this purpose? Check all that apply? 1. A gene X cDNA.
2. A DNA segment containing a fly promoter.
3. An enhancer active only in the antenna.
4. The enhancer of gene X.
5. A DNA segment containing the P element inverted repeat sequence.
6. Genomic DNA 5' and 3' to the transcription start of gene X.

Ms. w, a 21-year-old woman, came into a clinic after suffering a deep laceration on her foot while walking barefoot around her yard. the wound was cleaned, sutured, and bandaged, and she was released to return home after receiving tetanus antitoxoid. within 72 hours, the wound area was red and swollen, the suture line was dark in color, and it was accompanied by severe throbbing pain. ms. w had a high fever, her heart felt like it was racing, and she was finding it hard to concentraten even on simple tasks. she returned to the clinic and was immediately taken to the hospital. following lab tests, a diagnosis of acute necrotizing fasciitis was made. discussion questions 1. explain why ms. w. received a tetanus antitoxoid before leaving the hospital. (see chapters 3 and 4, infection and passive immunity.) 2. explain how acute necrotizing fasciitis developed in this case and the pathophysiology involved. (see acute necrotizing fasciitis.) 3. what is the potential outcome for ms. w if antibiotic drugs do not reduce the infection quickly?