Describe 2 limitations of a 3D cell model.

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Traditional Pathology: Traditional pathology involves removing cell culture models from well plates and processing them. Despite the fact that this method may obtain pathological information from tissues, the scale of these models makes the process extremely low-throughput, time-consuming, and costly.

Dissolution Based Assays: Many researchers are currently using dissolution based assays where cell culture models are homogenized and analyzed using a fluorescence probe such as Promega’s CellTiter-Glo Luminescent kit. While these assays are high-throughput, they do not capture the 3D data held within these models which makes them intrinsically more valuable than traditional 2D cell culture models.

3D structures is the fact that “spheres” can be formed, not from a single cell, but from a few cell clusters. However, even structures created as aggregates of several cells still have a three-dimensional form and seem to be a better model than flat, adherent cultures

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