A lab technician has four strains of bacteria. Although he forgot to label them, he knows that they include one F + strain, one Hfr strain, one F' strain, and one F - strain. He observed the following results after plating these strains on different media. Which of the following plates can be used to identify the F- strain?
strain his glu cys leu str
F+ + + + - +
Hfr + + + + -
F' + + + + -
F- - - - + +
a. minimal media with histidine, glutamate, and cysteine
b. minimal media with streptomycin
c. minimal media with leucine and streptomycin
d. complete media
e. minimal media with histidine, glutamate, cysteine, and streptomycin
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