In research, phalloidin can be used to fluorescently label actin filaments within a cell. a. Briefly describe how this is possible, considering that phalloidin itself is not fluorescent. a. What is the purpose of the fixation step?
b. What is the purpose of the permeabilization step?
c. How staining with AO and DAPI is different form staining with phalloidin?
d. Why you think DAPI and other fluorescent dyes that label nuclei are commonly used in labeling protocols?

Sally and sue were investigating the topic of friction in science. they used a small car and a ramp as seen in the picture to test what they were learning. they knew that they slipped easily on waxed floors but not on carpet, so they decided to change the material on the surface of the ramp to see what happened. they planned to use glass, carpet, aluminum foil, and sandpaper and run the car down the ramp over each surface. what would be the best research question to guide the girls' experiment? a) does the amount of surface area affect the friction on the moving car? b) will the car travel fastest on the glass surface? c) how does the angle of the ramp affect the speed of the car? d)do rougher surfaces tend to create more friction than smooth surfaces?

If a human has 23 pairs of chromosomes in every muscle cell of its body how many chromosomes will be in a human egg or sperm

Ascientist is designing an investigation to study the impact of invasive bird populations on local, native bird populations at backyard bird feeders. what population is most likely to be a control group for the investigation? amount of food number of bird feeders invasive bird populations local backyard birdfeeders without food