15. Assuming the fluorescence threshold is set to be 1. Which gene has the highest gene expression rate? What is the Ct value? Gene with highest expression = Ct value =

The correct answer is - Gene 1 and 14.5 (or 14).

Explanation:

The cycle threshold is denoted as Ct value. It is the cross point at the threshold line meets with fluorescence signal reaction curve. In the given RTpcr experiment, the threshold line is fluorescence intensity 1 thus, Ct value for each gene will be the point at which each curve meets the fluorescence intensity line at 1. Ct value of is inversely proportional to amount of nucleic acid in the given sample.

Here, the Ct for gene 1 is the lowest, so the amount of DNA would be highest.

Gene with highest expression= Gene 1

Ct value= 14.5

if the type of the food available changes, then the frequency of beak types will change, because birds with beaks more suited to the available food will be more successful over time.

explanation:

the universal gravitation equation. since the gravitational force is directly proportional to the mass of both interacting objects, more massive objects will attract each other with a greater gravitational force. if the mass of one of the objects is doubled, then the force of gravity between them is doubled.