Suppose a researcher introduces a mutation into the glucosidase domain of the mammalian glycogen debranching enzyme. The mutation inhibits the activity of the glucosidase but does not affect the other functions of the enzyme. The researcher then introduces the mutated enzyme into mammalian cells that do not express wild type glycogen debranching enzyme. Predict the effect of the mutation on glycogen metabolism.

cells will have glycogen molecules with branches of a single glucose residue

Explanation:

Glycogen debranching enzymes are proteins that catalyze the removal of branching from glycogen, which is a multibranched polysaccharide composed of glucose units and serves as a form of energy storage. These enzymes have two catalytic activities on a single polypeptide chain: glucosyltransferase and glucosidase activities, which are both required for glycogen breakdown. Glycogen degradation requires both glycogen phosphorylase and glycogen debranching enzymes. First, a glycogen phosphorylase acts to digest a glycogen branch down to four glucose residues. Subsequently, the debranching enzyme through its glucosyltransferase activity acts to transfer three glucose residues to a nearby branch of the glycogen chain. Finally, through its glucosidase activity, the debranching enzyme cleaves the remaining α-1,6 linkage in order to release free glucose.

answer:   option a) glucagon was secreted by his pancreas before he ate the cookies because blood glucose was low. insulin was secreted after he ate the cookies because his blood glucose was high.

explanation:

1. loamy sand is about 80% sand with 10% clay and defined mostly by sand particles with some clay to provide structure and fertility. the sand particles are easily visible and the soil holds structure but breaks apart easily.

2. scott sand can improve his soil quality by incorporating more silt and organic matter into the soil. peat moss and organic matter can also be incorporated.