The expression and purification of rGFP started with the growth of the bacterial culture and ended with the elution of rGFP off the Ni 2-agarose column. Assuming the column was not overloaded, which of the following changes to the procedure would result in an increase in the yield of rGFP in the elution fractions? A. Use a 1ml bed volume instead of a 0.5ml bed volume.
B. Induce the culture longer than 3 hours before harvesting.
C. Increase the number of washes before switching to elution buffer.
D. Use a 20ml pellet instead of a 15 ml pellet.