9. What is a two point calibration method? In your answer, include the term "buffer" and the pH of the two solutions used to calibrate the probe.

Aprimary producer stores 9,000 kcal. in the energy pyramid, if a primary producer is consumed by a first-level consumer, and that first-level consumer is consumed by a second-level consumer and the second-level consumer is consumed by a third-level consumer, how much energy is stored by the third-level consumer from the primary producer? *