You have a cell count of 6.7 x 105 cells / ml in a total volume of 4.5ml. You have resuspended your cells in 5ml sterile PBS. You need to seed a six-well plate with the following numbers: a. 2 wells at 1 x 104 cells/well (2ml/well)
b. 2 wells at 4 x 104 cells / well (2ml/well)
c. 2 wells at 2 x 10 cells / well (2ml / well)
Determine the volume of the original culture you will need to seed each concentration.