HELP HELP HELP (the last onne was deleted )

Why was the sample heated in step 5? describe the chemical principles involved in the reasoning behind this step?

Coat color in cats is determined by genes at several different loci. at one locus on the x chromosome, one allele (x ) encodes black fur and another allele (xo) encodes orange fur. females can be black (x x ), orange (xoxo), or a mixture of orange and black called tortoiseshell (x xo). males are either black (x y) or orange (xoy). bill has a female tortoiseshell cat named patches. one night, patches escapes from bill\'s house, spends the night out, and mates with a stray male. patches later gives birth to the following kittens: one orange male, one black male, two tortoiseshell females, and one orange female. what are the genotypes of patches, the stray male, and the kittens?

The nervous system interacts with the endocrine system by a. using hormones as connections b. sending nerve impulses directly to glands c. using neuroendocrine cells as connections d. sending neurotransmitters directly to neurons