Help with these 3 please

Cultures of the lake of the ozarks water samples were grown on enrichment media for identification. however, these organisms are present to some degree in all samples. an important question from a clinical perspective is whether or not there is a significant increase in the fecal coliform populations that would put people's health at risk. if you were part of the team investigating this outbreak, how would you best enumerate the fecal coliform threat? view available hint(s) cultures of the lake of the ozarks water samples were grown on enrichment media for identification. however, these organisms are present to some degree in all samples. an important question from a clinical perspective is whether or not there is a significant increase in the fecal coliform populations that would put people's health at risk. if you were part of the team investigating this outbreak, how would you best enumerate the fecal coliform threat? direct microscopic counts filtration count culture serial dilution count culture plate count culture

In what for do plants utilize nitrogen? why do they need nitrogen?

Name the part of the the brain labeled "e".