CollegeBoard AP Classroom
Unit 3 Progress one
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Researchers investigated the influence of environmental pH on the activity of peroxidase, an enzyme that catalyzes the conversion of hydrogen peroxide to water and oxygen gas. In an experiment
the researchers added a hydrogen peroxide solution containing guniacol to several identical test tubes and adjusted the solution in each test tube to a different pH. The researchers included the
guaiacol because it caused the solutions to change color as the reactions proceeded, which the researchers relied on for measuring reaction rates. Finally, the researchers added the same amount
of peroxidase to each test tube and measured the rate of each reaction at 23C. The results of the experiment are represented in Figure 1.
Relative Activity
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nH