Question 1 - i) In this experiment from Sallusto et al. 1994, "Two subsets of memory T lymphocytes with distinct homing potential and effector functions", what method was used to generate these data? Select only ONE answer. Justify your answer. a. Flow cytometry b. Radioactive thymidine incorporation and ELISA of culture supernatants c. Hemagglutination assay d. RT-PCR e. Southern blot Question 2 - ii) In this experiment from Sallusto et al. 1994, "Two subsets of memory T lymphocytes with distinct homing potential and effector functions", which of the following treatments was a negative control? Select only ONE answer. Justify your answer. a. CD45RA+CCR7+ naïve T cells (empty and filled squares) b CD45RA-CCR7+ memory T cells (empty and filled triangles) c. CD45RA-CCR7- memory T cells (empty and filled circles) d. There was no negative control in this experiment