You have just isolated a new drug that has only one effect on cell metabolism; it totally inhibits the breakdown of pre-rRNA to ribosomal RNA. After treating a culture of mammalian cells with this drug, you give the cells [3H]uridine for two minutes, and then grow the cells in the presence of the drug in unlabeled medium for four hours before extracting the RNA and centrifuging it through a sucrose gradient.
Required:
Draw the curves you would obtain by plotting both absorbance at 260 nm and radioactivity against the fraction number of the gradient.
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You have just isolated a new drug that has only one effect on cell metabolism; it totally inhibits t...
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