IN the lab you will prepare a dye solution to test transmembrane transport into yeast cells. The dye stock solution has a concentration of 5 mM. For your experiment, you want the dye concentration to be at 0.25 mM. You do the dilution with water. Select among the following, which method(s) will give you the correct final concentration of dye?
1) adding 1 ml of stock solution to 19 ml of water.
2) making a 25-fold dilution.
3) adding 100 ml of water to 5 ml of dye stock solution.
4) diluting 3 ml of concentrated dye with water to a final volume of 60 ml.

A All methods are accurate
B only methods 1, 2 and 3 are accurate
C only methods 1, 3 and 4 are accurate
D only methods 1 and 4 are accurate
E only method 3 is accurate
F None of the methods reflect the proper dilution method.