When performing experiments to measure the kcat of an enzyme, the substrate concentration should be: ?

The substrate concentration should be saturated as this is important in performing experiments in measuring Kcat of an enzyme for a saturated substrate concentration is needed because having a saturated substrate is a way of having more water or moisture in which is essential in this type of experiment in the substrate.

depends where

on my head

yes

on my legs

yes

on my

arms

yesh

the answer would be 3