Which of the following will cause an enzyme to permanently lose its properties
i. hydrolysis
ii. freezing to –20°c
iii. dissolving it in water
a. i only
b. ii only
c. i and ii only
d. i and iii only

you didn't give any options but heres what i can tell you: an inference is likely to change based on additional observations. the latter is something that you have seen or sensed using your sensory organs while the former is like a conclusion out from what you have observed.