Effects of increasing concentration of enzyme (lysozyme) on initial velocity (vo). Construct vo vs [E] (mg) plot. 2. The effects of increasing concentration of substrate (bacterial cell wall) on initial velocity (vo). a) Construct vo vs [S] plot and estimate the Km and Vmax. b) Construct Lineweaver-Burk (1/vo vs 1/[S]) and determine the Km and Vmax 3. Explain briefly the significance of Km and Vmax. 4. Effects of increasing temperature on initial velocity. Construct vo vs Temperature (OC) plot.
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