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Biology, 06.04.2021 03:20 ameliaparry123p2eupk
The 3000-base-pair (3 kb) vector contains a unique EcoRI site and a HindIII site 20 base pairs away. Your 1000-base-pair-long cDNA also contains a HindIII site 200 base pairs from the start. In the correct orientation, the initiating methionine codon (ATG) is closer to the promoter relative to the incorrect orientation. After cutting the plasmid with HindIII, what sizes (in base pairs) are expected for plasmids containing a cDNA in the correct orientation for protein expression
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