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Biology, 22.03.2021 16:20 ari10184

What are vegan and lacto ovo-vegiterian​

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Biology, 21.06.2019 20:00
You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false
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Biology, 21.06.2019 20:30
For your initial isolation of the bacterium, you simply needed to grow enough of it that you could identify some of its more general features. thus, you used a complex nutrient agar that would allow for the growth of almost any bacterial species. different bacterial species often have very specific requirements for life. this is a fact that you can utilize when you are trying to identify the bacterium to the species level. growth conditions that will permit the growth of one species but not another may allow you to select which one you would like to culture. you can utilize different selective/differential media that allow for the growth of only one type of bacteria (e.g., either gram positive or gram negative). culturing media such as phenylethyl alcohol or sodium chloride agar will be selective/differential for gram-positive bacteria. you may also want to show that it is different from other bacteria that may be present, so you could use a selective/differential agar. selective/differential media such as blood agar or phenol red sugar broths allow for the determination of whether a bacterial cell can grow and utilize specific ingredients included in the medium and turn the agar a distinct color. sheep's blood agar utilizes red blood cells to differentiate which bacterial species may have hemolytic properties.selective or differential? 1. an agar that uses a high salt concentration to limit the growth of one type of bacteria over another would be considered selective/differential. 2. if an agar contains a dye or ph indicator in the presence of a sugar, it is generally considered selective/differential for those bacteria that may ferment the sugar over those that cannot. 3. an agar that uses the dye in crystal violet agar suppresses the growth of gram-positive bacteria. this agar would be considered selective/differential. 4. the selective/differential aspect of macconkey agar allows for the determination of which bacteria are lactose fermenters and which are not. 5. a blood agar plate contains sheep red blood cells and allows for the determination of hemolytic capabilities for all bacteria that grow on the agar. this would make this type of agar selective/differential. 6. emb agar uses lactose and two dyes that allow it to be selective/differential between the blue-black colonies of e. coli and the pinkish colonies of all other enteric bacteria. 7. bile salts in macconkey agar allow for the agar to be selective/differential for the growth of enteric gram-negative bacteria over gram-positive bacteria. 8. an agar that allows for the distinction of bacteria based on metabolism would be considered selective/differential.
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Biology, 22.06.2019 19:00
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