Oxaloacetate is a key intermediate in the citric acid cycle, but it is also needed as a precursor for the synthesis of glucose during gluconeogenesis. Oxaloacetate is also fairly chemically unstable and undergoes spontaneous decarboxylation to pyruvate. Although oxaloacetate is not depleted by participation in the citric acid cycle, it can become depleted by other processes, necessitating its replenishment by various synthetic pathways. Use the Metabolic Map to identify enzymes that can replenish oxaloacetate or other citric acid cycle intermediates.

Carboxylating pyruvate into oxaloacetate.

Explanation:

Replenishing oxaloacetate is done by carboxylating pyruvate into oxaloacetate through a process that is catalyzed by an enzyme called pyruvate carboxylase. This enzyme speed up the process and the replenishing of oxaloacetate occur quickly and the oxaloacetate is ready to function and participate in different chemical reactions.

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