Each transfer RNA carries a specific amino acid. Since there are 20 different amino acids, the minimum number of transfer RNAs needed for protein synthesis is

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small, big, or no change could occur in the phenotype. it could cause something as simple as a cats ears to curl backwards or something as frighting as death. it could also occur in the protien-coding area and have an effect on that but no effect on the amino acids. what has to occur is a changing in the hox gene or the control gene.

not necessarily. higher fitness requires the organism to be reproductive successful. for example, an organism at age 10 with 15 offspring is much more fit than an organism at age 15 with only 5 offspring.