What occurs at step 3 in the diagram? O The restriction enzymes cut the isolated DNA into fragments.
The gene of interest is inserted into an expression cassette.
The recombinant DNA is inserted into the host cell nucleus via a vector.
O The expression cassette is inserted into a plasmid for cloning.

Use this dichotomous key for insect identification to identify the insect shown. 1. a. insect has one pair of wings. order diptera b. insect has two pairs of wings. go to #2 2. a. front wings thicker in texture than hind wings go to #3. b. front and hind wings are same texture throughout. go to #4 3. a. front wings are short order dermaptera b. front wings cover entire abdomen order coleoptera 4. a. wings with scale on all parts of their area. order lepidoptera b. wings without scales go to #5. 5. a. hind wings smaller than front wings. order ephemeroptera b. front and hind wings nearly equal in size. order odonata the insect pictured is in the order diptera. ephemeroptera. coleoptera. odonata.