Refer to the experimental set-up above. What is the purpose of the overturned bucket (note it is solid, not clear) in part A?

Protein and polypeptide sequences can be determined by first partially hydrolyzing the protein or polypeptide followed by amino acid sequencing of the fragment pieces using the chemical degradation process known as edman degradation. determine the sequence of the initial (18-amino acid) polypeptide that produces the following five partial sequences after hydrolysis and edman degradation.