This model shows the basic process of making a protein. What are the strengths and limitations of this model?

Results

We systematically analyze and compare how different modelling methodologies can be used to describe translation. We define various statistically equivalent codon-based simulation algorithms and analyze the importance of the update rule in determining the steady state, an aspect often neglected. Then a novel probabilistic Boolean network (PBN) model is proposed for modelling translation, which enjoys an exact numerical solution. This solution matches those of numerical simulation from other methods and acts as a complementary tool to analytical approximations and simulations. The advantages and limitations of various codon-based models are compared, and illustrated by examples with real biological complexities such as slow codons, premature termination and feedback regulation. Our studies reveal that while different models gives broadly similiar trends in many cases, important differences also arise and can be clearly seen, in the dependence of the translation rate on different parameters. Furthermore, the update rule affects the steady state solution.

Conclusions

The codon-based models are based on different levels of abstraction. Our analysis suggests that a multiple model approach to understanding translation allows one to ascertain which aspects of the conclusions are robust with respect to the choice of modelling methodology, and when (and why) important differences may arise. This approach also allows for an optimal use of analysis tools, which is especially important when additional complexities or regulatory mechanisms are included. This approach can provide a robust platform for dissecting translation, and results in an improved predictive framework for applications in systems and synthetic biology.

hope it help friends

interphase

the period of cell growth when the cell synthesizes new molecules and organelles. the cell is not dividing and it constitutes the majority of the time spent in the cell cycle

prophase

changes occur in the nucleus and cytoplasm. the chromatin condenses to form structures (sister chromatids) visible with a light microscope and the mitotic spindle begins to form, but the nucleus is still in tact

prometaphase

the nuclear envelope fragments and the spindle microtubules attach to the kinetochores of the sister chromatids

metaphase

all the cell's duplicated chromosomes are lined up at an imaginary plane equidistant between the poles of the mitotic spindle

anaphase

begins when sister chromatids separate from each other and ending when a complete set of daughter chromosomes arrives at each of the two poles of the cell

telophase

the daughter nuclei form at the two poles of a cell

cytokinesis

the division of a cytoplasm to form two separate daughter cells. usually occurs during telophase during mitosis. this and mitosis make up to mitotic (m) phase of the cell cycle

here is the answer

water, ice, acids, salts, plants, animals, and changes in temperature are all agents of weathering. weathering is the breaking down or dissolving of rocks and minerals on earths surface. once a rock has been broken down, a process called erosion transports the bits of rock and minerals away.