Mutated gene
DNA sequence: TAC-ATA-ACG-CCC-AGC-G
mRNA:
Codons:
Amino acid sequence:

mRNA: AUG-UAU-UGC-GGG-UCG-C

Codons: AUG, UAU, UGC, GGG, UCG

Amino acid sequence: Methionine- Tyrosine- Cysteine- Glycine- Tryptophan

Explanation:

This question is trying to illustrate the process of gene expression, which includes transcription (DNA - mRNA) and translation (mRNA - protein). According to this question, a mutated DNA sequence was given as follows: TAC-ATA-ACG-CCC-AGC-G

After transcription, which employs the complementary base pairing rule i.e A-U, G-C, the following mRNA sequence will be produced:

mRNA: AUG-UAU-UGC-GGG-UCG-C

After translation, which uses mRNA codons (a group of three nucleotide bases that encode a specific amino acids), the following amino acid sequence will be produced:

Amino acid sequence: Methionine (Met)- Tyrosine (Tyr)- Cysteine (Cys)- Glycine (Gly)- Tryptophan (Try).

Note that:

AUG encodes methionine

UAU encodes tyrosine

UGC encodes Cysteine

GGG encodes Glycine

UCG encodes tryptophan

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

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