Throughout the reflection, make sure you have a copy of the Student Guide You transcribed DNA to make mRNA and built a sequence of amino acids through. In your experiment, you tested this prediction: tRNA lipids translation mRNA Prediction: RNA determines the sequence of amino acids in proteins and polypeptides by a two-step process: transcription of DNA produces mRNA in the nucleus, then translation of the mRNA to tRNA takes place in the ribosome in the cytoplasm.