You have cloned a gene for an enzyme that degrades lipids in a bacterium that normally lives in cold temps. you wish to use this gene in e. coli to produce industrial amounts of enzyme for use in laundry detergent.
a. how would you accomplish this?
b. you have managed to produce transgenetic e. coli expressing mrna of your gene, but only a low level of protein is produced. why might this be so? how can you overcome this problem?

A) You would have to incorporate that gene into the bacteria's plasmid (genetic information that isn't in its main genome) so that it can use it and express its message. This is done using enzymes that cut that circular plasmid, insert the gene you want, and put the circular molecule back together. Once you have the bacteria with the plasmid in it, you replicate that bacteria, so all the resulting copies will have that gene and they'll express it.

B) If not enough protein is being produced it could be because you don't have enough bacteria, you'd need a bigger population. The medium the bacteria is in also should be optimal so that it can be as efficient as possible.

No that answer is wrong. you have written the function of mitochondria in chloroplast and function of chloroplast in mitochondria.

Proteins are the workhorses of the cell, controlling virtually every reaction within as well as providing structure and serving as signals to other cells. proteins are long chains of  amino acids  , and the exact sequence of the amino acids determines the final structure and function of the protein. instructions for that sequence are encoded in  genes  . to make a particular protein, a messenger ribonucleic acid (mrna) copy is made from the gene (in the process called  transcription  ), and the mrna is transported to the  ribosome  . protein synthesis, also called  translation  , begins when the two ribosomal subunits link onto the mrna. this step, called initiation, is followed by elongation, in which successive amino acids are added to the growing chain, brought in by transfer rnas (trnas). in this step, the ribosome reads the  nucleotides  of mrna three by three, in units called  codons  , and matches each to three nucleotides on the trna, called the anticodon. finally, during termination, the ribosome unbinds from the mrna, and the  amino acid  chain goes on to be processed and folded to make the final, functional protein