g Why are affinity tags usually placed at the amino or carboxy terminus of a protein instead of somewhere in the middle?

The arrangements of lipids in the membrane makes the cell semi permeable .

Ithink due to shortage of water

You should provide a picture to show where the arrow is pointing and which information that is given. but there are 3 branches of the government, legislative, executive, and judicial. legislative makes laws, executives pass laws, and judicial are the courts