What is the most efficient procedure that scientists can use to obtain more recombinant DNA for a particular trait? isolate more recombinant DNA
use electrophoresis to separate more recombinant DNA
clone the recombinant DNA in a biological vector
use restriction enzymes to cut the recombinant DNA into fragments

Technique that allows DNA to be combined from different sources; also called gene or DNA splicing. Recombinant DNA is an important technique for many gene-cloning applications.

1) Isolate plasmid: probe is used to isolate the gene of interest

2) Enzymatically cleave DNA into fragments: restriction enzyme that can locate and cut the gene from the DNA segment, cuts an opening in the recipient DNA (usually a plasmid) where the donor DNA can be attached

3) Isolate fragment w/ gene of interest: DNA ligase attaches donor and recipient DNA, forming a recombinant DNA molecule

4) Insert gene into plasmid: recombinant DNA molecule is inserted into host cell

5) Insert plasmid & gene into bacterium

6) Culture bacteria

The CORRECT answer is C.) clone the recombinant DNA in a biological vector.

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