Biology, 20.10.2020 20:01 zuleromanos

Protein X is a secreted protein produced as a precursor form called pro-X. The maturation of Protein X requires that a short N-terminal segment be removed (propeptide cleaving). You have generated two antibodies that are called Anti-Pro and Anti-C. Anti-Pro binds to sequence in the N-terminal segment of pro-X, but only when pro-X is intact. Anti-C binds the C-terminal half of Protein X. You want to use these antibodies to determine where, within the vesicle trafficking pathway, propeptide cleavage occurs. You perform a cell fractionation procedure and obtain five test tubes containing different cellular compartments (Fractions 1-5). In addition to Anti-Pro and Anti-C, you also probe the fractions with antibodies to clathrin and COP-II proteins. Your results are summarized in the following Table. (2 points each, 6 points total) Reaction of fraction with specific antibody: Anti-Pro Anti-C Anti-COPII Anti-clathrin Fraction 1 Fraction 2 Fraction 3 Fraction 4 +- Fraction 5 (+ = reacts; +++ = strongly reacts; - = no detectable reaction; +/- = reacts weakly) + + +++ +++ + +++ +++ A. From this data, which fraction contains: cis-Golgi: plasma membrane: ER: trans-Golgi:
B. In which fraction (1-5) is the spring protein likely to be undergoing processing (cleavage) into its mature form and briefly explain WHY?
C. What does fraction 2 represent and WHY?

Answers: 3

Show answers

Another question on Biology

Biology, 21.06.2019 16:00

Many kangaroo rats live in the sonoran desert of the southwestern united states. they have a variety of adaptations for living in the desert. under which circumstance would the kangaroo rats of the sonoran desert be most likely to develop new adaptations by natural selection?

Answers: 1

Answer

Biology, 21.06.2019 16:30

Which of the following is not a main characteristic that scientists focus on when determining which kingdom an organism belongs to

Answers: 3

Answer

Biology, 22.06.2019 05:00

Which statements about the fluid mosaic structure of a membrane are correct? select the three correct statements.which statements about the fluid mosaic structure of a membrane are correct? select the three correct statements.the framework of a membrane is a bilayer of phospholipids with their hydrophilic heads facing the aqueous environment inside and outside of the cell and their hydrophobic tails clustered in the center.because membranes are fluid, membrane proteins and phospholipids can drift about in the membrane.the diverse proteins found in and attached to membranes perform many important functions.the kinky tails of some proteins keep the membrane fluid by preventing the component molecules from packing solidly together.membranes include a mosaic, or mix, of carbohydrates embedded in a phospholipid bilayer.

Answers: 1

Answer

Biology, 22.06.2019 05:40

Benito is doing research on the effects of florida's elevation on its climate. his notes include the following: 1. the average elevation of florida is 30 m above sea level. 2. the highest point in florida is britton hill, which is 105 m above sea level. 3. the change in temperature from 0 m to 1,000 m is 7 °c. what can benito conclude about the effects of florida's elevation on its climate? a.) florida typically experiences cold temperatures and temperature changes within a narrow range. b.) florida typically experiences cold temperatures and temperature changes within a broad range. c.) florida typically experiences warm temperatures and temperature changes within a narrow range. d.) florida typically experiences warm temperatures and temperature changes within a broad range.

Answers: 1

Answer

You know the right answer?

Protein X is a secreted protein produced as a precursor form called pro-X. The maturation of Protein...

Questions