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Biology, 21.09.2020 15:01 chjam265

Just when the Novel Prize looms clearly on the horizon, tragedy strikes: the reaction ceases to work well. The only difference between when the reaction was working and now is that the DNA polymerase is from a different source (isolated from a different organism). [NOTE: enzymes like polymerases used in most laboratories are purchased in kits from companies.] The Km of the "old" polymerase was 20 µM nucleotide, and the Km of the "new" polymerase is 100 µM. What is the first thing you would do to change the reaction conditions (using the new polymerase) in your attempt to get the reaction to work?

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